Galactokinase activity in Streptococcus thermophilus
نویسندگان
چکیده
منابع مشابه
Galactokinase activity in Streptococcus thermophilus.
ATP-dependent phosphorylation of [14C]galactose by 11 strains of Streptococcus thermophilus indicated that these organisms possessed the Leloir enzyme, galactokinase (galK). Activities were 10 times higher in fully induced, galactose-fermenting (Gal+) strains than in galactose-nonfermenting (Gal-) strains. Lactose-grown, Gal- cells released free galactose into the medium and were unable to util...
متن کاملCharacterization of a galactokinase-positive recombinant strain of Streptococcus thermophilus.
The lactic acid bacterium Streptococcus thermophilus is widely used by the dairy industry for its ability to transform lactose, the primary sugar found in milk, into lactic acid. Unlike the phylogenetically related species Streptococcus salivarius, S. thermophilus is unable to metabolize and grow on galactose and thus releases substantial amounts of this hexose into the external medium during g...
متن کاملCloning and characterization of the galactokinase gene from Streptococcus thermophilus.
The objective of this research was to clone and characterize the galactokinase gene (galK) from Streptococcus thermophilus F410. Partially digested genomic DNA was cloned into pBR322 and transformed into galK Escherichia coli, and a galactose-fermenting transformant was isolated. Restriction analysis revealed that the transformant resulted from a Sau3A-HindIII 4.0-kb fragment. Galactokinase act...
متن کاملStreptococcus thermophilus.
Streptocus tmophilus, as a species designation (Orla-Jensen, 1919), has included those members of the nonhemolytic streptococci that possess the unusual combination of characteristics of extreme salt sensitivity, nonobligate thermophily, a rigorous fermentative pattern with respect to certain sugars (Sherman, 1937), and, based upon attempts to culture on synthetic media, a notoriety for nutriti...
متن کاملDiversity, activity, and evolution of CRISPR loci in Streptococcus thermophilus.
Clustered regularly interspaced short palindromic repeats (CRISPR) are hypervariable loci widely distributed in prokaryotes that provide acquired immunity against foreign genetic elements. Here, we characterize a novel Streptococcus thermophilus locus, CRISPR3, and experimentally demonstrate its ability to integrate novel spacers in response to bacteriophage. Also, we analyze CRISPR diversity a...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1985
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.50.4.777-780.1985